EXPERIENCE AND RESULTS OF HPV TESTING OF WOMEN IN THE MOSCOW REGION
T.S. Budykina, V.I. Krasnopolsky, N.V. Zarochentseva, L.K. Dzhidzhikhia, N.S. Menshikova,
O.V. Trishchenkova, M.N. Minina, E.V. Pelshe
For quotation:
Budykina T.S., Krasnopolsky V.I., Zarochentseva N.V. etc. Experience and results of HPV testing
women of the Moscow region. Questions of practical colposcopy. Genital infections. 2022; (1): 28–31.
DOI 10.46393/27826392_2022_1_28
Annotation:
Purpose of the study: to determine the infection of women in the Moscow region with the human papillomavirus of high carcinogenic risk (HPV HCR).
Material and methods. HPV testing was carried out on 2970 women in the Moscow region aged 16 to 90 years. Using the real-time PCR method (“AmpliPrime® HPV VKR 14” and “AmpliPrime® HPV VKR 14 genotype”), 14 HPV genotypes of high carcinogenic risk were determined (16, 18, 31, 33, 35, 39, 45, 51, 52, 56 , 58, 59, 66, 68). All women with established HPV infection underwent a cytological examination with interpretation of the results according to the Bethesda classification (The Bethesda system, TBS, 2001). Statistical processing and calculation of data were carried out using Microsoft Excel and GraphPad Prism 8.0 software.
Results. HPV HPV DNA was detected in 25.8% of women examined (766/2970). The most common type was HPV type 16, which accounted for about 41.5% (318/766) of all HPV-positive patients. When assessing the results of traditional cytology in HPV-positive patients, pathological signs were detected in 40.7% (211/519) of cases in the group of women under 30 years of age: LSIL - 12.7% (66/519), HSIL - 21.4% (111/519), ASCUS – 0.2% (1/519), ASC-H – 3.9% (20/519), AGC-NOS – 2.5% (13/519). In the group of women over 30 years old, pathological smears accounted for 37.7% (93/247), their distribution was as follows: LSIL - 21.9% (54/247), HSIL - 10.1% (25/247), ASCUS - 3.6% (9/247), ASC-H – 1.6% (4/247), AGC-NOS – 0% and AGC-FN – 0.4% (1/247).
Conclusion. HPV testing by PCR followed by cytological examination provides
large coverage of examined women with the identification of a significant number of cases of precancerous lesions
in different age groups. Introduction of HPV typing as primary screening for cervical cancer
will not only improve the results of detecting cervical pathology, but also increase the inter-screening
interval up to 5 years.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
T.S. Budykina, V.I. Krasnopolsky, N.V. Zarochentseva, L.K. Dzhidzhikhia, N.S. Menshikova,
O.V. Trishchenkova, M.N. Minina, E.V. Pelshe
For quotation:
Budykina T.S., Krasnopolsky V.I., Zarochentseva N.V. etc. Experience and results of HPV testing
women of the Moscow region. Questions of practical colposcopy. Genital infections. 2022; (1): 28–31.
DOI 10.46393/27826392_2022_1_28
Annotation:
Purpose of the study: to determine the infection of women in the Moscow region with the human papillomavirus of high carcinogenic risk (HPV HCR).
Material and methods. HPV testing was carried out on 2970 women in the Moscow region aged 16 to 90 years. Using the real-time PCR method (“AmpliPrime® HPV VKR 14” and “AmpliPrime® HPV VKR 14 genotype”), 14 HPV genotypes of high carcinogenic risk were determined (16, 18, 31, 33, 35, 39, 45, 51, 52, 56 , 58, 59, 66, 68). All women with established HPV infection underwent a cytological examination with interpretation of the results according to the Bethesda classification (The Bethesda system, TBS, 2001). Statistical processing and calculation of data were carried out using Microsoft Excel and GraphPad Prism 8.0 software.
Results. HPV HPV DNA was detected in 25.8% of women examined (766/2970). The most common type was HPV type 16, which accounted for about 41.5% (318/766) of all HPV-positive patients. When assessing the results of traditional cytology in HPV-positive patients, pathological signs were detected in 40.7% (211/519) of cases in the group of women under 30 years of age: LSIL - 12.7% (66/519), HSIL - 21.4% (111/519), ASCUS – 0.2% (1/519), ASC-H – 3.9% (20/519), AGC-NOS – 2.5% (13/519). In the group of women over 30 years old, pathological smears accounted for 37.7% (93/247), their distribution was as follows: LSIL - 21.9% (54/247), HSIL - 10.1% (25/247), ASCUS - 3.6% (9/247), ASC-H – 1.6% (4/247), AGC-NOS – 0% and AGC-FN – 0.4% (1/247).
Conclusion. HPV testing by PCR followed by cytological examination provides
large coverage of examined women with the identification of a significant number of cases of precancerous lesions
in different age groups. Introduction of HPV typing as primary screening for cervical cancer
will not only improve the results of detecting cervical pathology, but also increase the inter-screening
interval up to 5 years.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
