Squamous intraepithelial lesions of the cervix: is there a connection with the microbiome?
G.R. Bayramova, A.V. Peremykina, A.O. Andreev, D.A. Dobrovolskaya
For quotation:
Bayramova G.R., Peremykina A.V., Andreev A.O., Dobrovolskaya D.A. Squamous intraepithelial lesions of the cervix: is there a connection with the microbiome? Questions of practical colposcopy. Genital infections. 2022; (1): 60–65.
DOI 10.46393/27826392_2022_1_60
Annotation:
The article presents a systematic analysis of the results of modern clinical studies on the problem of a disrupted microbiome and transcriptome in women with cervical intraepithelial neoplasia (CIN). Moderate to severe CIN (CIN II–III) may precede the development of cervical cancer (CC) for several years or even decades. Currently, many risk factors are known that contribute to the development of cervical CIN and cervical cancer. At the same time, according to a number of authors, the microbiome of the vagina and cervical canal plays a major role in the development and progression of CIN and cervical cancer. Thus, at present, timely detection and treatment of cervical intraepithelial lesions associated with intraepithelial lesions is especially important.
opportunistic mixed infections. From these positions, bacterial vaginosis is considered as an acute problem in gynecological practice, affecting the incidence of precancerous diseases of the cervix.
The study results showed the importance of detailed analysis of the vaginal microbial community using next generation sequencing (NGS). These studies were carried out using the SNP method, based on the analysis of bacterial 16S rRNA genes, which has high diagnostic accuracy and allows us to determine the diversity of the microbial landscape.
A study of the transcriptome in women with CIN showed changes in many microRNA molecules, which, with further study, can become markers for CIN and CC. The introduction of SNP into laboratory diagnostic methods will make it possible to reliably identify altered expression of microRNAs associated with the progression of CIN to cervical cancer.
Conclusion. Studying the microbiome of the vaginal biotope and the cervical canal will identify groups of patients at risk for the progression of precancerous lesions of the cervix and cervical cancer. Transcriptome studies have shown changes in many microRNA molecules (SALL4, FOXO1, HBD-1, HBD-2, HBD-3, LL37, psoriasin, IL-8, etc.) in women with CIN and CC.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
G.R. Bayramova, A.V. Peremykina, A.O. Andreev, D.A. Dobrovolskaya
For quotation:
Bayramova G.R., Peremykina A.V., Andreev A.O., Dobrovolskaya D.A. Squamous intraepithelial lesions of the cervix: is there a connection with the microbiome? Questions of practical colposcopy. Genital infections. 2022; (1): 60–65.
DOI 10.46393/27826392_2022_1_60
Annotation:
The article presents a systematic analysis of the results of modern clinical studies on the problem of a disrupted microbiome and transcriptome in women with cervical intraepithelial neoplasia (CIN). Moderate to severe CIN (CIN II–III) may precede the development of cervical cancer (CC) for several years or even decades. Currently, many risk factors are known that contribute to the development of cervical CIN and cervical cancer. At the same time, according to a number of authors, the microbiome of the vagina and cervical canal plays a major role in the development and progression of CIN and cervical cancer. Thus, at present, timely detection and treatment of cervical intraepithelial lesions associated with intraepithelial lesions is especially important.
opportunistic mixed infections. From these positions, bacterial vaginosis is considered as an acute problem in gynecological practice, affecting the incidence of precancerous diseases of the cervix.
The study results showed the importance of detailed analysis of the vaginal microbial community using next generation sequencing (NGS). These studies were carried out using the SNP method, based on the analysis of bacterial 16S rRNA genes, which has high diagnostic accuracy and allows us to determine the diversity of the microbial landscape.
A study of the transcriptome in women with CIN showed changes in many microRNA molecules, which, with further study, can become markers for CIN and CC. The introduction of SNP into laboratory diagnostic methods will make it possible to reliably identify altered expression of microRNAs associated with the progression of CIN to cervical cancer.
Conclusion. Studying the microbiome of the vaginal biotope and the cervical canal will identify groups of patients at risk for the progression of precancerous lesions of the cervix and cervical cancer. Transcriptome studies have shown changes in many microRNA molecules (SALL4, FOXO1, HBD-1, HBD-2, HBD-3, LL37, psoriasin, IL-8, etc.) in women with CIN and CC.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
