MODERN CONCEPTS ABOUT THE ROLE OF NOD GENE POLYMORPHISM IN THE PATHOGENESIS OF TUBAL INFERTILITY IN PATIENTS WITH A PAST CHLAMYDIAL INFECTION
Dubrovina S.O., Ardintseva O.A., Mashkina E.V.
For quotation:
Dubrovina S.O., Ardintseva O.A., Mashkina E.V. Modern ideas about the role of NOD gene polymorphism
in the pathogenesis of tubal infertility in patients with previous chlamydial infection. Practical questions
colposcopy. Genital infections. 2022; (1): 78–81.
DOI 10.46393/27826392_2022_1_78
Annotation:
Purpose of the study: to improve the management of patients with tubal-peritoneal infertility after chlamydial infection based on the study of polymorphic variants rs6958571 of the NOD1 gene (A>C) and s2066847 of the NOD2 gene (CCC>CC).
Material and methods. 93 patients of reproductive age with infertility were divided into groups depending on the infertility factor. Subgroup 1A consisted of 32 women with tubal infertility and fallopian tube pathology, subgroup 1B included 25 women with a history of chlamydial infection and the absence of fallopian tube pathology during diagnostic laparoscopy. The control group included 35 patients with infertility of unspecified origin without pathology of the fallopian tubes and no history indicating a previous chlamydial infection. To isolate DNA from peripheral blood, a commercial reagent kit, Genomik DNA Mini Kit PureLink (Invitrogen by ThermoFisher Scientific), was used. Gene polymorphism analysis was carried out using TaqMan MGB probes using real-time polymerase chain reaction (TaqMan® SNP Genotyping Assays, Thermo Fisher
Scientific). Statistical processing of the results was carried out using the Statistica 12.5 application package.
Results. There were no statistically significant differences in the frequency of the rs6958571 polymorphism of the NOD1 gene (A>C) between the subgroups of group 1 (p > 0.05) and in comparison of the control group with subgroups (group 2 vs subgroup 1A (p > 0.05) and group 2 vs subgroup 1B (p > 0.05)). When analyzing rs2066847 of the NOD2 gene (CCC>CC), there were also no statistically significant differences between the subgroups of group 1 (p > 0.05) and in comparison of the control group with subgroups (group 2 vs subgroup 1A (p > 0.05) and group 2 vs subgroup 1B (p > 0.05)).
Conclusion. Thus, the absence of statistically significant differences in the frequency of registration of polymorphism of the NOD1, NOD2 genes between patients with a history of chlamydial infection and pathology of the fallopian tubes and without it, as well as when compared with patients with unspecified infertility and normal fallopian tubes without a history of STIs is not allows us to confirm the previously put forward assumption that variations in these genes may be responsible for ascending chlamydial infection and, as a consequence, tuboperitoneal infertility.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
Dubrovina S.O., Ardintseva O.A., Mashkina E.V.
For quotation:
Dubrovina S.O., Ardintseva O.A., Mashkina E.V. Modern ideas about the role of NOD gene polymorphism
in the pathogenesis of tubal infertility in patients with previous chlamydial infection. Practical questions
colposcopy. Genital infections. 2022; (1): 78–81.
DOI 10.46393/27826392_2022_1_78
Annotation:
Purpose of the study: to improve the management of patients with tubal-peritoneal infertility after chlamydial infection based on the study of polymorphic variants rs6958571 of the NOD1 gene (A>C) and s2066847 of the NOD2 gene (CCC>CC).
Material and methods. 93 patients of reproductive age with infertility were divided into groups depending on the infertility factor. Subgroup 1A consisted of 32 women with tubal infertility and fallopian tube pathology, subgroup 1B included 25 women with a history of chlamydial infection and the absence of fallopian tube pathology during diagnostic laparoscopy. The control group included 35 patients with infertility of unspecified origin without pathology of the fallopian tubes and no history indicating a previous chlamydial infection. To isolate DNA from peripheral blood, a commercial reagent kit, Genomik DNA Mini Kit PureLink (Invitrogen by ThermoFisher Scientific), was used. Gene polymorphism analysis was carried out using TaqMan MGB probes using real-time polymerase chain reaction (TaqMan® SNP Genotyping Assays, Thermo Fisher
Scientific). Statistical processing of the results was carried out using the Statistica 12.5 application package.
Results. There were no statistically significant differences in the frequency of the rs6958571 polymorphism of the NOD1 gene (A>C) between the subgroups of group 1 (p > 0.05) and in comparison of the control group with subgroups (group 2 vs subgroup 1A (p > 0.05) and group 2 vs subgroup 1B (p > 0.05)). When analyzing rs2066847 of the NOD2 gene (CCC>CC), there were also no statistically significant differences between the subgroups of group 1 (p > 0.05) and in comparison of the control group with subgroups (group 2 vs subgroup 1A (p > 0.05) and group 2 vs subgroup 1B (p > 0.05)).
Conclusion. Thus, the absence of statistically significant differences in the frequency of registration of polymorphism of the NOD1, NOD2 genes between patients with a history of chlamydial infection and pathology of the fallopian tubes and without it, as well as when compared with patients with unspecified infertility and normal fallopian tubes without a history of STIs is not allows us to confirm the previously put forward assumption that variations in these genes may be responsible for ascending chlamydial infection and, as a consequence, tuboperitoneal infertility.
Journal “Issues of practical colposcopy. Genital infections" No. 1_2022
https://drive.google.com/file/d/1yqJO9cj6B3sqyJXUjGbdrXYKLQ3KHBPW/view
